Similarity of Helicobacter pylori isolated from drinking water and peptic ulcer patients.

Author(s)

A.A.AL-Sulami , A.A.AL-Abdula , T.A.A.AL-Edani ,

Download Full PDF Pages: 15-21 | Views: 1008 | Downloads: 240 | DOI: 10.5281/zenodo.3408009

Volume 2 - March 2013 (03)

Abstract

In this study two stomach biopsy samples from antrum were obtained from each of 110 patients. One sample is for rapid urease test and the other for bacterial culture. The results showed that rapid urease test (RUT) was positive in 46(41.8%) cases, while in culturing method, H.pylori was isolated from 68(62%) based on oxidase, urease, catalase and Gram stain. There were no significant differences (P>0.05) of H.pylori among males and females. The highest detection rates of the bacterium , (80.76 %) was recorded in the age group 31- 40 years , while no case was recorded in the age group below 20 years. Statistically, the differences were significant (p<0.05) among these age groups. The higher percentage of resistance was against erythromycin (93%) for H.pylori isolated from biopsy and (100%) for H.pylori isolated from water, whereas the lowest percentage of resistance was against tetracycline (20%) for H.pylori isolated from biopsy and (29%) from water samples. There are no significant differences (P>0.05) between drinking water and biopsy to the antibiotics.16SrRNA PCR was used for the confirmation of isolated H.pylori . PCR results showed the presence of the 500 bp 16SrRNA sequence in 8 out of 15 tested clinical isolates. Similarity between clinical and water isolates, obtained from a previous study is indicated by RFLP using restriction enzyme (EcoRI) for whole DNA. This bears an epidemiological significance as it indicates a possible source of this pathogen outside the human stomach

Keywords

Helicobacter pylori, drinking water , peptic ulcer patients

References

  1. Marshall, B. and Warren, J.R. (1983). Unidentified curved bacilli on gastritis. Lancet, 2: 1273-1275.
  2. NIH: National Institute of Health Consensus Conference. (1994). Helicobacter pylori in peptic ulcer disease. JAMA, 272: 65-69.
  3. Blaser MJ, Atherton JC (February 2004). "Helicobacter pylori persistence: biology and disease". J. Clin. Invest. 113 (3): 321–33.
  4. Elitsur Y, Yahav J. (2005). Helicobacter pylori infection in El-Zaatari F. A., Nguyen A. M., Genta R. M., Klein P. D., Graham D. Y. Determination of Helicobacter pylori status by reverse transcription-polymerase chain reaction. Comparison with urea breath test. Dig. Dis. Sci. 1995; 40: 109–113.
  5. Kusters JG, van Vliet AH, Kuipers EJ. (2006). Pathogenesis of Helicobacter pylori ori infection. Clin Microbiol Rev., 19: 449-490.
  6. Kivi, M., and Y. Tindberg. (2006). Helicobacter pylori occurrence and transmission: a family affair? Scand. J. Infect. Dis., 38:407–417.
  7. Azevedo, N. F., C. Almeida, L. Cerqueira, S. Dias, C. W. Keevil, and M. J. Vieira. (2007). Coccoid form of Helicobacter pylori as a morphological manifestation of cell adaptation to the environment. Appl. Environ. Microbiol., 73:3423–3427.
  8. Klein, P. D., D. Y. Graham, A. Gaillour, A. R. Opekun, and E. O. Smith for the Gastrointestinal Physiology Working Group. (1991). Water source as risk factor for Helicobacter pylori infection in Peruvian children. Lancet 337:1503–1506.
  9. Hulten, K., S. W. Han, H. Enroth, P. D. Klein, A. R. Opekun, R. H. Gilman, D. G. Evans, L. Engstrand, D. Y . Graham, and F. A. el-Zaatari. (1996). Helicobacter pylori in the drinking water in Peru. Gastroenterology 110: 1031–1035.
  10. Hulten, K.; Enorth, H.; Nystrom, T. and Engstrand, L. (1998). Presence of Helicobacter species DNA in Swedish water. J. Appl. Microbiol. 85:282-286.
  11. Horiuchi, T., T. Ohkusa, M. Watanabe, D. Kobayashi, H. Miwa, and Y.Eishi. (2001). Helicobacter pylori DNA in drinking water in Japan. Microbiol.Immunol,. 45:515–519
  12. Bunn, J. E., W. G. MacKay, J. E. Thomas, D. C. Reid, and L. T. Weaver.(2002). Detection of Helicobacter pylori DNA in drinking water biofilms: implications for transmission in early life. Lett. Appl. Microbiol. 34:450–454.
  13. Benson, J. A., K. A. Fode-Vaughan, and M. L. Collins. (2004). Detection of Helicobacter pylori in water by direct PCR. Lett. Appl. Microbiol., 39:221– 225.
  14. Krumbiegel, P., I. Lehmann, A. Alfreider, G. J. Fritz, D. Boeckler, U. Rolle- Kampczyk, M. Richter, S. Jorks, L. Muller, M. W. Richter, and O. Herbarth. (2004). Helicobacter pylori determination in non-municipal drinking water and epidemiological findings. Isotopes Environ. Health Stud., 40:75–80
  15. Watson, C. L., R. J. Owen, B. Said, S. Lai, J. V. Lee, S. Surman-Lee, and G.Nichols. (2004). Detection of Helicobacter pylori by PCR but not culture in water and biofilm samples from drinking water distribution systems in England.J. Appl. Microbiol., 97:690–698. American Based Research Journal Vol-2-Issue-3 Feb-2013 ISSN (2304-7151)
  16. Mazari-Hiriart, M., Y. Lopez-Vidal, S. Ponce-de-Leon, J. J. Calva, F. Rojo- Callejas, and G. Castillo-Rojas.( 2005). Longitudinal study of microbial diversity and seasonality in the Mexico City metropolitan area water supply system. Appl. Environ. Microbiol., 71:5129–5137.
  17. Al-Sulami, A.A., Al-Edani, T.A.A., Al-Abdula, A.A. (2012). Culture method and PCR for the detection of Helicobacter pylori in drinking water in Basrah Governorate Iraq. J. Gastroenterology Research and Practice.
  18. Hegarty, J. P., M. T. Dowd, and K. H. Baker. (1999). Occurrence of Helicobacter pylori in surface water in the United States. J. Appl. Microbiol. 78:697–701.
  19. Beneduce, L.; Spano, G.; Libergoli, M.; Labonia, M. and Massa, S. (2003). Survival of Helicobacter pylori in well water. World J. Microbiol. Biotechnol. 19:505-508.
  20. Al-Sulami, A.A., Al-Kiat, H.S., Bakker, L.K. and Hunnon, H. (2008). Primary isolation and detection of Helicobacter pylori from dyspeptic patients : a simple, rapid method. East Meditter Health J;Mar-Ap,14(2) 268-76.
  21. AL-Sulami, A.A.; AL-Taee, A. and Jumaa, M. (2010). Isolation and identification of Helicobacter pylori from drinking water in Basrah governorate. Accepted in the EMHJ
  22. Baron, J.D.; Fingold, M.S. and Peterson, R.C. (1994). Dignostic th Microbiology.9 ed. Mosby. Your book Jnc.:440-443.
  23. Piddock, L.J.J. ( 1991). A review techniques use for the determination of antimicrobial resistances and sensitivity in bacteria. Appl. Bacteriol. 68, 307-318.
  24. Falsafi, T.; Favaedi, R.; Mahjoub, F. and Najafi, M.( 2009).Application of Stool-PCR test for diagnosis of Helicobacter pylori infection in children. World. J. Gastroenterol. 15(4): 484-488 .
  25. Peek, R. M., Miller, G. G., Tham, K. T., Perez-Perez, G. I., Cover, T. L., Atherton, J. C., et.al. (1995). Detection of Helicobacter pylori gene expression in human gastric mucosa. J. Cln. Microbiol. 33(1): 28-32.
  26. Axon, A.T.R. (1996). The transmission of Helicobacter pylori: which theory fits the facts? Eur. J. Gastroent. Hepatol. 8:1-2.
  27. Vaira, D.; Ferron, P.; Negrinin, R.; Cavazzini, L.; Holton, J.; Ainley,C.; Londei, M.; Vergura, M.; Dei, R. and Colecchia, A. (1992). Detection of Helicobacter pylori-like organisms in the stomach of some food-source animals using a monoclonal antibody. Ital. J. Gastroenterol. 24:181-184.
  28. Otto, G., S. H. Hazell, J. G. Fox, C. R. Howlett, J. C. Murphy, J. L. O’Rourke, and A. Lee. (1994). Animal and public health implications of gastric colonization of cats by Helicobacter-like organisms. J. Clin. Microbiol. 32:1043–1049.
  29. Hopkins, R. J., P. A. Vial, C. Ferreccio, J. Ovalle, P. Prado, V. Sotomayor, R. G. Russel, S. S. Wasserman, and J. G. Morris. (1993). Seroprevalence of Helicobacter pylori in Chile—vegetables may serve as one route of transmission. J. Infect. Dis. 168:222–226.
  30. Shimizu, T., T. Akamatsu, H. Ota, and T. Katsuyama. (1996). Immunohistochemical detection of Helicobacter pylori in the surface mucous gel layer and its clinicopathological significance. Helicobacter 1:197–206.
  31. Genta, R. M., R. M. Huberman, and D. Y. Graham. (1994). The gastric cardia in Helicobacter pylori infection. Hum. Pathol. 25:915–919.
  32. Louw, J. A., V. Falck, C. van Rensburg, J. Zak, G. Adams, and I. N. Marks. (1993). Distribution of Helicobacter pylori colonization and associated gastric inflammatory changes: difference between patients with duodenal and gastric ulcers. J. Clin. Pathol. 46:754–756.
  33. Azevedo, N. F., Almeida, C.; Fernandes, I.; Cerqueira, L.; Dias, S.; Keevil, C. W. and Vieira, M. J. (2008). Survival of Gastric and Enterohepatic Helicobacter spp. in Water: Implications for Transmission. Appl. Environ. Microbiol., 74:1805–1811.
  34. Lee, A. and Megraud, F. (1996). Helicobacter pylori: techniques for clinical diagnosis and basic research
  35. Brandi, G., B. Biavati, C. Calabrese, M. Granata, A. Nannetti, P. Mattarelli, G. Di Febo, G. Saccoccio, and G. Biasco. (2006). Urease-positive bacteria other than Helicobacter pylori in human gastric juice and mucosa. Am. J.Gastroenterol. 101:1756–1761. (First published 16 June 2006; doi:10.1111/ j.1572-0241.2006.00698.x.)
  36. Meurer, L. N. and Bower, D. J. (2002). Management of Helicobacter pylori infection. J.American Family Physician. 65, 7:
  37. Andre Dubois. (1995). Spiral bacteria in the human stomach: Gastric Helicobacter pylori. J. EID.1 (3): 8-15.
  38. Ugwuga, E. I., and Ugwu, N. C., (2007). Helicobacter pylori in Uninvestigated Dyspepsia In primary cares in Abakaliki, Nigeria. J. Health and Aplid Science.6 (1).
  39. Bernstein, C. N.; McKeown , I.; Embil, J. M.; Blanchard, J. F.; Dawood, M.; Kabani, A.; Kliewet, E.; Smart, G.; Coghlan, G.; MaeDonald, S.; Cook, C. and Orr, P. (1999). Seroprevalence of Helicobacter pylori, incidence of gastric cancer, and peptic ulcer-associated hospitalizations in a Canadian Indian population. Dig. Dis. Sci. 44: 668-674.
  40. Nijevitch, A.A.; Sataev, V.U.; Vakhitov, V.A.; et al.( 2001). Childhood peptic ulcer in the Ural area of Russia: clinical status and Helicobacter pylori-associated immune response. Pediatr. Gastroenterol. Nutr.33(5):558-64
  41. Lu, Y., Redlinger, T.E., Avitia, R., Galindo, A., Goodman, K., (2002). Isolation and genotyping of Helicobacter pylori from untreated municipal wastewater. Appl. Environ. Microbiol. 68, 1436–1439.
  42. Mobley, H.L.T.; Mends, G.L. and Hasell,S.L.(2001). Helicobacter pylori physiology and genetics. ASM Press, Washington, D.

Cite this Article: